Andrew McMahon (PI)
University of Southern California
We will extend our hGUDMAP studies of the human fetal kidney to generate new resources for the RBK consortium. In a collaboration with ABCAM, we will characterize rabbit monoclonal antibodies for cross species (mouse and human) cell-type specificity in identifying key cell types of the developing kidney. We will validate MARIS (Method for Analyzing RNA following Intracellular Sorting) as a broadly applicable approach for acquiring transcriptional signatures from specific cell types in the developing kidneys. Finally, recognizing the importance of Six2 in regulating nephron progenitors, we will compare the Six2 regulatory landscape in the embryonic kidney with that of pluripotent stem cell derived Six2+ nephron progenitors generated in cell culture.