Molecular Characterization of Normal and Stem Cell Derived Kidney Cell Types

Key Personnel

Andrew McMahon (PI)
University of Southern California

• Jill McMahon
  University of Southern California
• Albert Kim
  University of Southern California
• Nils Lindstrom
  University of Southern California
• Tracy Tran
  University of Southern California

Project Description

We will extend our hGUDMAP studies of the human fetal kidney to generate new resources for the RBK consortium. In a collaboration with ABCAM, we will characterize rabbit monoclonal antibodies for cross species (mouse and human) cell-type specificity in identifying key cell types of the developing kidney. We will validate MARIS (Method for Analyzing RNA following Intracellular Sorting) as a broadly applicable approach for acquiring transcriptional signatures from specific cell types in the developing kidneys. Finally, recognizing the importance of Six2 in regulating nephron progenitors, we will compare the Six2 regulatory landscape in the embryonic kidney with that of pluripotent stem cell derived Six2+ nephron progenitors generated in cell culture.