Human Kidney Biopsy Single Cell Protocols and Analysis

Key Personnel

Benjamin D. Humphreys (PI)
Washington University in St. Louis

Project Description

Our partnership project aims to establish conditions for the transportation and storage of human kidney biopsies. These protocols will be optimized to ensure maximal cell viability and preservation of mRNA integrity. In the second phase of this project, we will compare single cell RNA sequencing technologies: FACS-seq and Drop-seq. Our goal is to determine which approach will be best adapted to the large-scale investigation of human kidney disease in large populations.